abstract：:A rapid and simple DNA labeling system has been developed for disposable microarrays and has been validated for the detection of 117 antibiotic resistance genes abundant in Gram-positive bacteria. The DNA was fragmented and amplified using phi-29 polymerase and random primers with linkers. Labeling and further amplifi...

journal_title：Journal of microbiological methods

authors： Strauss C,Endimiani A,Perreten V

更新日期：2015-01-01 00:00:00

abstract：:Streptococcus agalactiae (GBS) is a leading cause of invasive neonatal infection. Serotyping of GBS is important in following epidemiological trends and vaccine development. Capsular serotyping of GBS by latex agglutination has been the predominant typing method, but more recently capsular genotyping has been introduc...

journal_title：Journal of microbiological methods

authors： Brigtsen AK,Dedi L,Melby KK,Holberg-Petersen M,Radtke A,Lyng RV,Andresen LL,Jacobsen AF,Fugelseth D,Whitelaw A

更新日期：2015-01-01 00:00:00

abstract：:Characterization of commercial microbial consortia products for human and environmental health risk assessment is a major challenge for regulatory agencies. As a means to develop an approach to assess the potential environmental risk of these products, research was conducted to compare four genomics methods for charac...

journal_title：Journal of microbiological methods

authors： Samarajeewa AD,Hammad A,Masson L,Khan IU,Scroggins R,Beaudette LA

更新日期：2015-01-01 00:00:00

abstract：:The development of biofilms in static plates was monitored. Glass coupons were placed on agar covered with filter paper, which was inoculated with suspended bacteria. The viable cell density, biofilms matrix and biomass were quantified. The method is excellent for adhesion and material studies, due to its simplicity a...

journal_title：Journal of microbiological methods

authors： Oja T,Blomqvist B,Buckingham-Meyer K,Goeres D,Vuorela P,Fallarero A

更新日期：2014-12-01 00:00:00

abstract：:The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armil...

journal_title：Journal of microbiological methods

authors： Longa CM,La Porta N

更新日期：2014-12-01 00:00:00

abstract：:Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I...

journal_title：Journal of microbiological methods

authors： Savar NS,Dashti A,Darzi Eslam E,Jahanian-Najafabadi A,Jafari A

更新日期：2014-11-01 00:00:00

abstract：:A variety of molecular typing techniques have been developed to investigate the clonal relationship among bacterial isolates, including those associated with nosocomial infections. In this study, the authors evaluated whole-genome mapping as a tool to investigate the genetic relatedness between Pseudomonas aeruginosa ...

journal_title：Journal of microbiological methods

authors： Boers SA,Burggrave R,van Westreenen M,Goessens WHF,Hays JP

更新日期：2014-11-01 00:00:00

abstract：:In this study, food-borne yeast isolates (n=96), comprising at least 33 species, were identified using MALDI-TOF MS and conventional methods (API ID 32 C and Phoenix Yeast ID). Discrepancies of both methods were resolved by sequencing the ITS1-5.8S-rRNA-ITS2 region. For ten isolates, mainly classified to Rhodotorula a...

journal_title：Journal of microbiological methods

authors： Pavlovic M,Mewes A,Maggipinto M,Schmidt W,Messelhäußer U,Balsliemke J,Hörmansdorfer S,Busch U,Huber I

更新日期：2014-11-01 00:00:00

abstract：:Site-directed mutagenesis is one of the most widely used techniques in life sciences. Here we describe an improved and simplified method for introducing mutations at desired sites. It consists of an inverse PCR using a plasmid template and two partially complementary primers. The synthesis step is followed by annealin...

journal_title：Journal of microbiological methods

authors： Füzik T,Ulbrich P,Ruml T

更新日期：2014-11-01 00:00:00

abstract：:Four different bacterial DNA extraction strategies and two different qPCR probe chemistries were studied for detection of Stapylococcus aureus from white cheeses. Method employing trypsin treatment followed by a commercial kit application and TaqMan probe-based qPCR was the most sensitive one detecting higher counts t...

journal_title：Journal of microbiological methods

authors： Kadiroğlu P,Korel F,Ceylan C

更新日期：2014-10-01 00:00:00

abstract：:Test and evaluation of engineered biothreat agent detection systems ("biodetectors") are a challenging task for government agencies and industries involved in biosecurity and biodefense programs. In addition to user friendly features, biodetectors need to perform both highly sensitive and specific detection, and must ...

journal_title：Journal of microbiological methods

authors： Berchebru L,Rameil P,Gaudin JC,Gausson S,Larigauderie G,Pujol C,Morel Y,Ramisse V

更新日期：2014-10-01 00:00:00

abstract：:A protein database was constructed and validated with identification rate over 90% for the 4 most common Gram-negative bacteria on agar plates. By protein masses comparison, 120 bacteria of the 4 species from blood culture bottles were identified. The concordance was high (Kappa=0.906) between our method and conventio...

journal_title：Journal of microbiological methods

authors： Xiao D,Yang Y,Jiang W,Zhang H,Liu H,Yu H,Xie C,Zhong M,Chen L,Huang W

更新日期：2014-10-01 00:00:00

abstract：:Using two-step-PCR screening which consists of direct and priority-based PCR and inverse PCR, fungal gene-deletion mutants were selected quickly and accurately. It omits genomic DNA extraction and Southern blotting steps and prevents misinterpretations caused by PCR failure. It is anticipated to facilitate large-scale...

journal_title：Journal of microbiological methods

authors： Park J,Kim S,Kwon S,Lee YH

更新日期：2014-10-01 00:00:00

abstract：:We report here the construction of a plasmid vector designed for the efficient electrotransformation of the periodontal pathogen Porphyromonas gingivalis. The novel Escherichia coli-Bacteroides/P. gingivalis shuttle vector, designated pTIO-1, is based on the 11.0-kb E. coli-Bacteroides conjugative shuttle vector, pVAL...

journal_title：Journal of microbiological methods

authors： Tagawa J,Inoue T,Naito M,Sato K,Kuwahara T,Nakayama M,Nakayama K,Yamashiro T,Ohara N

更新日期：2014-10-01 00:00:00

abstract：:Corynebacterium matruchotii is a microbial inhabitant in the oral cavity of humans and is associated with the formation of dental calculi. C. matruchotii forms highly specific morphological units, which are referred to as corn-cobs. Although other Corynebacterium species have frequently been isolated from the oral cav...

journal_title：Journal of microbiological methods

authors： Tsuzukibashi O,Uchibori S,Shinozaki-Kuwahara N,Kobayashi T,Takada K,Hirasawa M

更新日期：2014-09-01 00:00:00

abstract：:Extracellular DNA (eDNA) is a ubiquitous biological compound in aquatic sediment and soil. Previous studies suggested that eDNA plays an important role in biogeochemical element cycling, horizontal gene transfer and stabilization of biofilm structures. Previous methods for eDNA extraction were either not suitable for ...

journal_title：Journal of microbiological methods

authors： Alawi M,Schneider B,Kallmeyer J

更新日期：2014-09-01 00:00:00

abstract：:The European Union Reference Laboratory (EURL) for Listeria monocytogenes (Lm) collaborates with a network of 35 National Reference Laboratories (NRLs) throughout Europe. Most of these NRLs are in charge of detecting and typing Lm strains from food, environment and animals, which are isolated nationally. The past few ...

journal_title：Journal of microbiological methods

authors： Félix B,Danan C,Van Walle I,Lailler R,Texier T,Lombard B,Brisabois A,Roussel S

更新日期：2014-09-01 00:00:00

abstract：:Interest in using Bacillus strains as probiotic components of animal feeds has grown in recent years. However, some of these strains, especially those taxonomically related to the Bacillus cereus group, may have enterotoxigenic activity. Assessment of their toxigenic potential by well-established and robust protocols ...

journal_title：Journal of microbiological methods

authors： Blanch AR,Méndez J,Castel S,Reina M

更新日期：2014-08-01 00:00:00

abstract：:The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the M...

journal_title：Journal of microbiological methods

authors： Yang S,Liebner S,Alawi M,Ebenhöh O,Wagner D

更新日期：2014-08-01 00:00:00

abstract：:Mycoplasma synoviae, an important poultry pathogen, belonging to the class Mollicutes, causes airsacculitis, synovitis, decreased egg production and produces significant economic losses. Efforts to determine M. synoviae virulence factors and their role in pathogenicity require suitable tools for genetic manipulation o...

journal_title：Journal of microbiological methods

authors： Shahid MA,Marenda MS,Markham PF,Noormohammadi AH

更新日期：2014-08-01 00:00:00

abstract：:The food industry requires easy, accurate, and cost-effective techniques for microbial identification to ensure safe products and identify microbial contaminations. In this work, FTIR spectroscopy and MALDI-TOF mass spectrometry were assessed for their suitability and applicability for routine microbial diagnostics of...

journal_title：Journal of microbiological methods

authors： Wenning M,Breitenwieser F,Konrad R,Huber I,Busch U,Scherer S

更新日期：2014-08-01 00:00:00

abstract：:Blue native electrophoresis (BNE) has become the most popular method for the global analysis of membrane protein complexes. Although it has been shown to be very useful for that purpose, it can produce the dissociation of complexes with weak interactions and, due to the use of Coomassie Brilliant Blue, does not allow ...

journal_title：Journal of microbiological methods

authors： Diéguez-Casal E,Freixeiro P,Costoya L,Criado MT,Ferreirós C,Sánchez S

更新日期：2014-07-01 00:00:00

abstract：:A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enume...

journal_title：Journal of microbiological methods

authors： Trevathan-Tackett S,Macreadie P,Ralph P,Seymour J

更新日期：2014-07-01 00:00:00

abstract：:Now-a-days increasing emergence of antibiotic-resistant pathogenic microorganisms is one of the biggest challenges for management of disease. In the present study comparative genomics, metabolic pathways analysis and additional parameters were defined for the identification of 94 non-homologous essential proteins in S...

journal_title：Journal of microbiological methods

authors： Ghosh S,Prava J,Samal HB,Suar M,Mahapatra RK

更新日期：2014-06-01 00:00:00

abstract：:To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided importan...

journal_title：Journal of microbiological methods

authors： Zheng Y,Zhang H,Wang D,Gao P,Shan A

更新日期：2014-06-01 00:00:00

abstract：:In wastewater, biological phosphate removal can fail because of the presence of glycogen accumulating organism (GAO), therefore measuring glycogen stored in microbial cultures provides information on the bacterial population type. Once glycogen is hydrolysed to glucose it was accurately measured using a human glucose ...

journal_title：Journal of microbiological methods

authors： Flavigny R

更新日期：2014-05-01 00:00:00

abstract：:MALDI-TOF mass spectrometry (MALDI-TOF MS) is increasingly used as a reliable technique for species identification of bacterial pathogens. In this study we investigated the question of whether MALDI-TOF MS can be used for accurate sub-differentiation of strains and isolates of two important nosocomial pathogens Entero...

journal_title：Journal of microbiological methods

authors： Lasch P,Fleige C,Stämmler M,Layer F,Nübel U,Witte W,Werner G

更新日期：2014-05-01 00:00:00

abstract：:Central nervous system (CNS) infections have multiple potential causative agents for which simultaneous pathogen screening can provide a useful tool. This study evaluated a multiplexed microarray for the simultaneous detection of antibodies against CNS pathogens. The performance of selected microarray antigens for the...

journal_title：Journal of microbiological methods

authors： Jääskeläinen AJ,Viitala SM,Kurkela S,Hepojoki S,Sillanpää H,Kallio-Kokko H,Bergström T,Suni J,Närvänen A,Vapalahti O,Vaheri A

更新日期：2014-05-01 00:00:00

abstract：:I formulated a systematic approach to screen for colicin resistance-associated genes by using an attenuated colicin mutant and demonstrated its utility in a screen of genes related to colicin E5 resistance. Screening of an Escherichia coli genome library revealed rstA as a partial resistance gene to colicin E5. Transc...

journal_title：Journal of microbiological methods

authors： Futai K

更新日期：2014-05-01 00:00:00

abstract：:Microbiological work requires a reliable source of cultures that are not only well defined and taxonomically determined, but are also adequately preserved without changes in their morphological, physiological and genetic traits. Here we describe an easy, cost effective and rapid method for reliably preserving filament...

journal_title：Journal of microbiological methods

authors： Hu X,Webster G,Xie L,Yu C,Li Y,Liao X

更新日期：2014-04-01 00:00:00

abstract：:The Calgary Biofilm Device (CBD) has been described as a technology for the rapid and reproducible assay of biofilm susceptibilities to antibiotics. In this study a simple and inexpensive alternative to the CBD was developed from polypropylene (PP) microcentrifuge tubes and pipette tip boxes. The utility of the device...

journal_title：Journal of microbiological methods

authors： Almshawit H,Macreadie I,Grando D

更新日期：2014-03-01 00:00:00

abstract：:Coagulase negative staphylococci (CoNS) are a leading cause of infections in preterm infants, mostly involved in late-onset infection in low birth weight neonates. The epidemiology and pathophysiology of these infections remain unclear, notably because the causing agents are gathered in the artificial CoNS group. The ...

journal_title：Journal of microbiological methods

authors： Filleron A,Simon M,Hantova S,Jacquot A,Cambonie G,Marchandin H,Jumas-Bilak E

更新日期：2014-03-01 00:00:00

abstract：:Staphylococcus aureus remains a leading, virulent pathogen capable of expressing complex drug resistance that requires up to 2-4 days for laboratory analysis. In this study, we evaluate the ability of automated microscopy of immobilized live bacterial cells to differentiate susceptible from non-susceptible responses o...

journal_title：Journal of microbiological methods

authors： Price CS,Kon SE,Metzger S

更新日期：2014-03-01 00:00:00

abstract：:Microarrays have allowed gene expression profiling to progress from the gene level to the genome level, and oligonucleotide microarrays have become the platform of choice for large-scale, targeted gene expression studies. cDNA arrays and spotted oligonucleotide arrays have gradually given way to in situ synthesized ol...

journal_title：Journal of microbiological methods

authors： Venkataraman B,Vasudevan M,Gupta A

更新日期：2014-02-01 00:00:00

abstract：:Clostridium botulinum is the etiological agent of botulism. Due to food-borne poisoning and the potential use of the extremely toxic botulinum neurotoxin (BoNT) from C. botulinum in bioterror or biocrime related actions, reliable high resolution typing methods for discriminating C. botulinum strains are needed. Partia...

journal_title：Journal of microbiological methods

authors： Olsen JS,Scholz H,Fillo S,Ramisse V,Lista F,Trømborg AK,Aarskaug T,Thrane I,Blatny JM

更新日期：2014-01-01 00:00:00

abstract：:DNA probes specific for rRNA of selected target species were utilised for the detection of beer spoilage bacteria of the genera Pectinatus and Megasphaera using a hybridisation protection assay (HPA). All the probes were modified during synthesis by addition of an amino linker arm at the 5' end or were internally modi...

journal_title：Journal of microbiological methods

authors： Paradh AD,Hill AE,Mitchell WJ

更新日期：2014-01-01 00:00:00

abstract：:Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that ...

journal_title：Journal of microbiological methods

authors： Lartigue MF,Bouloc P

更新日期：2014-01-01 00:00:00

abstract：:The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing re...

journal_title：Journal of microbiological methods

authors： Schubert M,Stührk C,Fuhr MJ,Schwarze FW

更新日期：2013-11-01 00:00:00

abstract：:Phenotypic differentiation between Campylobacter fetus (C. fetus) subspecies fetus and C. fetus subspecies venerealis is hampered by poor reliability and reproducibility of biochemical assays. AFLP (amplified fragment length polymorphism) and MLST (multilocus sequence typing) are the molecular standards for C. fetus s...

journal_title：Journal of microbiological methods

authors： van der Graaf-van Bloois L,van Bergen MA,van der Wal FJ,de Boer AG,Duim B,Schmidt T,Wagenaar JA

更新日期：2013-10-01 00:00:00

abstract：:Exocellulases play a key role in cleaving the accessible ends of cellulose molecules to release soluble glucose and cellobiose. To date, there have been no screens for exocellulase owing to assay protocol limitations, the high cost of substrates, and low activity of exocellulases compared with endocellulases. This stu...

journal_title：Journal of microbiological methods

authors： Ko KC,Han Y,Cheong DE,Choi JH,Song JJ

更新日期：2013-09-01 00:00:00